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Image Search Results
Journal: Journal of neurochemistry
Article Title: Enhanced expression and shedding of the transmembrane chemokine CXCL16 by reactive astrocytes and glioma cells.
doi: 10.1111/j.1471-4159.2005.03123.x
Figure Lengend Snippet: Fig. 3 Induction of CXCL16 mRNA expression by proinflammatory cytokines in vitro. Cultured human endothelial cells and U343 glioma cells were stimulated with either 10 ng/mL of IFNc or TNFa alone or a combination of the two cytokines. After 24 h of incubation, the CXCL16 mRNA level was quantified by TaqMan RT-PCR and expressed as the percentage of that determined for the non-stimulated control. The expression of GAPDH mRNA was not affected by cell stimulation (not shown). Data are presented as means and SD of one representative of three independent experiments each performed in triplicates.
Article Snippet: Recombinant human CXCL16 extracellular domain and chemokine domain,
Techniques: Expressing, In Vitro, Cell Culture, Incubation, Reverse Transcription Polymerase Chain Reaction, Control, Cell Stimulation
Journal: Journal of neurochemistry
Article Title: Enhanced expression and shedding of the transmembrane chemokine CXCL16 by reactive astrocytes and glioma cells.
doi: 10.1111/j.1471-4159.2005.03123.x
Figure Lengend Snippet: Fig. 4 Expression and release of CXCL16 protein. (a) Glioma cells were left untreated or stimulated with IFNc and TNFa for 24 h. Sub- sequently, the cells were washed and cell extracts prepared with Tri- ton-X100 were analysed by Western blotting with a rabbit antibody against human CXCL16. As a control, extracts of COS7 cells trans- fected with hemagglutinin (HA)-tagged CXCL16 were analysed in parallel. The transfected CXCL16 was detected by both, an anti-HA and the anti-CXCL16 antibody. Broad bands of the glycosylated transmembrane form of CXCL16 are visible at 49 kDa, the expression is stimulated by cytokine treatment. (b) The human glioma cell line (U 343) and glioma cells cultivated from a grade IV surgical glioma (A 761) were treated with IFNc (10 ng/mL), TNFa (10 ng/mL) glutamate (10 mM), substance p (10 nM), EGF (10 ng/mL), TGFb1 (10 ng/mL) and HIL-6 (10 ng/mL), for 24 h, and subsequently conditioned media were harvested and analysed for the presence of soluble CXCL16 by ELISA. Data are shown as means and SD of one representative of three independent experiments each performed in triplicates.
Article Snippet: Recombinant human CXCL16 extracellular domain and chemokine domain,
Techniques: Expressing, Western Blot, Control, Transfection, Enzyme-linked Immunosorbent Assay